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KMID : 1177720090030020023
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Journal of Alternatives to Animal Experiments
2009 Volume.3 No. 2 p.23 ~ p.28
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Evaluation of inhibition effect of plant extract on the PPD induced skin sensitization in Dendritic cell surrogate model
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An Su-Sun
Kim Seo-Young
Jung Kyoung-Mi
Lim Kyung-Min
Lee Tae-Ryong
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Abstract
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The Council of the European Union has banned marketing cosmetics and its ingredients tested on animals since March 2009. Because of this regulation, various alternatives to animal experimentation to test efficacy or toxicity of cosmetics and other products have been developed. Evaluation of the skin sensitization potential is an important part of the safety assessment of new ingredients in cosmetics and drugs to be applied topically. In recent years, various in vitro alternative methods on the basis of DC maturation were postulated to identify the contact sensitizers. Because of availability and abundance, some monocytic leukemia cell lines (KG-1, THP-1, U-937, and MUTZ-3, etc) were proposed as in vitro dendritic cell differentiation models. Especially, the expressions of CD54 and CD86 on the human monocytic leukemia cell line, THP-1 are used as indicators for skin sensitization potential after intensive investigation as an in vitro alternative approach for the identification of contact sensitizers. We applied this test method to predict the possibility that Nelumbo nucifera root extract (P7TE) could inhibit paraphenylenediamine (PPD) induced sensitization. While PPD 90 μg/ml
induced CD54 expression on THP-1 cells, co-treatment of various concentrations of P7TE and PPD decrease the
augmentation of CD54 at the 350 μg/ml of P7TE. We also found similar suppressive effect in Local lymph
node assay (LLNA).
We suggest that sensitization test method using THP-1 cell could be also applied to screen the substances
mitigating allergic contact dermatitis and these substances could be used in cosmetics and quasi drugs.
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KEYWORD
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allergic contact dermatitis, PPD, inhibition effect, Nelumbo nucifera root extract
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